Regulation of polyamine transport in murine L1210 leukemia cells was characterized in order to better understand its relationship to specific intracellular polyarnines and their analogs and to quantitate the sensitivity by which it is controlled. Up-regulation of polyamine uptake was evaluated follo
Effects of polyamines and analogs on staphylococcal nuclease
β Scribed by H. Yanagawa; Y. Ogawa; Dr. F. Egami
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 347 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0233-111X
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β¦ Synopsis
The promoting activity of polyamine analogs (IV -XV) on staphylococcal nuclease with DNA as the substrate was compared with that of natural polyamines (I -111): I. NH,(CH,),NH(CH,),NH(CH,),NH2 (spermine) ; 11. NH,(CH,),NH(CH,),NH(CH,),NH, (thermine) ; 111. NH,(CH,),NK, (putrescine);
π SIMILAR VOLUMES
ments showed some decrease in cleavage, which indicates the participation of redox processes.
Several mutant forms of staphylococcal nuclease with one or two defined amino acid substitutions have been purified, and the effects of the altered amino acid sequence on the stability of the folded conformation have been analyzed by guanidine hydrochloride denaturation. Two nuc-mutations, which gre
The equilibrium folding pathway of staphylococcal nuclease (SNase) has been approximated using a statistical thermodynamic formalism that utilizes the highresolution structure of the native state as a template to generate a large ensemble of partially folded states. Close to 400,000 different states