Regulation of Myelin Basic Protein (Arginine) Methyltransferase by Thyroid Hormone in Myelinogenic Cultures of Cells Dissociated from Embryonic Mouse Brain
โ Scribed by Shashi G. Amur; Gouri Shanker; Ronald A. Pieringer
- Book ID
- 111172683
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 500 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0022-3042
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๐ SIMILAR VOLUMES
The developmental pattern of the myelin-associated 5'-nucleotidase and its regulation by L-3,3',5,-triiodothyronine (T,) have been demonstrated in a culture system of cells dissociated from embryonic mouse brain. Hypothyroid calf serum containing low levels of T3 (31 ng/100 ml), and thyroxine, T4 (<
Sinefungin, a known inhibitor of protein methylation, inhibited the myelin basic protein (arginine) methyltransferase activity in homogenates of cultured cerebral cells from embryonic mice. Fifty percent inhibition was achieved with 25pM sinefungin. Electron microscopic examination of the myelin fra
The concentration of myelin basic protein (MBP) mRNA in primary cultures of cells dissociated from embryonic mouse cerebra and grown in the presence of varyin amounts of thyroid hormone was measured using a 3FiP-labeled cDNA probe and a dot-blot procedure. The cDNA probe contained 1.85 kilobases of
Primary cultures of cells dissociated from embryonic mouse brain were demonstrated to be a useful system for studying cell proliferation and its regulation. Ornithine decarboxylase activity was closely correlated with the rate of DNA and RNA synthesis during cell growth. suggesting that the enzyme i