Investigations on myelinogenesis in vitro: Developmental expression of myelin basic protein mRNA and its regulation by thyroid hormone in primary cerebral cell cultures from embryonic mice
✍ Scribed by G. Shanker; A. T. Campagnoni; Dr. R. A. Pieringer
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 470 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0360-4012
No coin nor oath required. For personal study only.
✦ Synopsis
The concentration of myelin basic protein (MBP) mRNA in primary cultures of cells dissociated from embryonic mouse cerebra and grown in the presence of varyin amounts of thyroid hormone was measured using a 3FiP-labeled cDNA probe and a dot-blot procedure. The cDNA probe contained 1.85 kilobases of the gene for MBP. The concentration of mRNA specific for MBP in control cells grown on a medium containing normal (euthyroid) calf serum increased with increasing age of culture. The greatest increase occurred between 15 and 35 days in culture (5.25-fold increase); whereas between 35 and 50 days in culture, the rate of accumulation slowed to yield a net increase of MBP mRNA of only 10%. The quantity of MBP mRNA was drastically diminished at all ages studied when the cells were grown from the sixth day onward on a medium containing hypothyroid calf serum. Although the amount of MBP mRNA in hypothyroidtreated cells did increase, the change in concentration was less (3.43-fold), and it peaked earlier (at 30 days). dependent on thyroid hormone during a critical period early in the life of the individual Flynn et al, 19771. The question of how thyroid hormone regulates myelination is perhaps best studied in primary cultures of brain cells where the interaction between hormone and target cells can be better controlled than in the intact animal. Primary cultures of embryonic mouse cerebra, which produce myelin and have an ontogenetic pattern that mimics that of the whole animal, have been particularly useful in uncovering a number of myelin-specific parameters that are sensitive to thyroid and other hormone manipulation [