Ornithine decarboxylase (ODC, EC 4.1.1.17) activity is induced in the RAW264 macrophage-like cell line by bacterial lipopolysacharide (LPS). As little as 0.1 ng/ml LPS promoted an increase in ODC activity, while maximal ODC activity (30-fold above control) was induced with 1.0 pg/ml LPS. An increase
Regulation of mouse ornithine decarboxylase gene expression in a macrophage-like cell line: Synergistic induction by bacterial lipopolysaccharide and camp
โ Scribed by Shuang Zheng; Christine M. McElwain; Steven M. Taffet
- Book ID
- 117055418
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 401 KB
- Volume
- 175
- Category
- Article
- ISSN
- 0006-291X
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Ornithine decarboxylase (ODC) activity was rapidly induced in the RAW264 macrophage-like cell line after treatment with bacterial lipopolysaccharide (LPS). ODC mRNA levels were determined by isolating cellular RNA, followed by Northern blot and dot blot analysis using a 32P-labeled cDNA probe. ODC m
The induction of tumor necrosis factor a (TNF-a) by polytetrafluoroethylene (PTFE) particles (5-50 pm) and by bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA) was examined in RAW cell cultures. Twenty-four-hour culture supernatants from the treated and control cells were assayed for TN