Synergistic induction of tumor necrosis factor ? by bacterial lipopolysaccharide and lipoteichoic acid in combination with polytetrafluoroethylene particles in a murine macrophage cell line RAW 264.7

✍ Scribed by Chapekar, Mrunal S. ;Zaremba, Terrye G. ;Kuester, Robert K. ;Hitchins, Victoria M.

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 529 KB
• Volume: 31
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199606)31:2<251::aid-jbm12>3.0.co;2-o

✦ Synopsis

The induction of tumor necrosis factor a (TNF-a) by polytetrafluoroethylene (PTFE) particles (5-50 pm) and by bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA) was examined in RAW cell cultures. Twenty-four-hour culture supernatants from the treated and control cells were assayed for TNF-a using a mouse L929 cell cytotoxicity assay. Untreated RAW cells produced low levels of endogenous TNFa in the culture supernatants. Addition of 0.5 ng to 1 p g / mL LPS or 1 ng to 1 pg/ml LTA increased the TNF-a production by 7-3570-fold and 2-815-fold, respectively. Addition of 1-5 mg PTFE increased the TNF-a production by 6-17fold over the untreated control cell levels. The cells exposed to PTFE and 0.5 ng/mL LPS or 5 ng/mL LTA produced TNFa levels that were significantly higher than those produced by any inducer alone. Thus, both LTA, a Gram-positive bacterial cell wall component and LPS, a Gram-negative bacterial cell wall component, can induce TNF-a production, which is further enhanced by PTFE particles in RAW cells.