Regulation of intercellular adhesion molecule-1 expression by retinoic acid: Analysis of the 5′ regulatory region of the gene

✍ Scribed by Fawzi Aoudjit; Marc Bosse; Christian Stratowa; Günter Voraberger; Marie Audette

• Book ID: 102868028
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: French
• Weight: 920 KB
• Volume: 58
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910580416

✦ Synopsis

and 2Ernst Boehringer lnstitut, Bender and Co., fienna, Austria.

MATERIAL AND METHODS

Chemicals and cell culture

Retinoic acid and PMA were purchased from Sigma (St. Louis, MO). Retinoic acid was prepared weekly as a 10-mM stock solution in absolute ethanol and kept in the dark at -20°C. FBS was obtained from Hyclone (Logan, UT). Enzymes were purchased from Pharmacia/LKB (Montreal, Canada). Radiochemicals were from Amersham (Oakville, Canada) or Dupont New England Nuclear (Mississauga, Canada). The human neuroblastoma cell line SK-N-SH was obtained from the ATCC (HTB 11) and maintained in DMEM supplemented with 10% FBS, 2 mM glutamine and 35 pg/ml gentamycin. Media were changed twice a week, and cells were passaged on a weekly basis.