Regulation of immunoglobulin gene expression in trans by phorbol esters

✍ Scribed by Inga-Lill Mårtensson; Antonio Iglesias; Tomas Leanderson

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 878 KB
• Volume: 19
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830190823

✦ Synopsis

Regulation of immunoglobulin gene expression in trans by phorbol esters*

Lipopolysaccharide-stimulated B cell cultures treated with phorbol-12,13-dibutyrate have been shown to down-regulate their steady state levels of ps RNA while the pm RNA remained constant. In contrast, the steady state levels of 6 RNA was upregulated by the same treatment. By using mice transgenic for a rearranged p or 6 gene, it could be shown that the down-regulatory effect acts in trans on a defined DNA stretch and is independent of isotype. Thus, the positive effect on endogenous 6 RNA steady-state levels must be regulated at the level of RNA processing. In addition, a DNA construct containing 1.5 kb of a x promoter 5' of, and an IgH enhancer 3' of, a chloramphenicol acetyl transferase reporter gene could be down-regulated in trans by anti-w or phorbol-12,13-dibutyrate treatment, showing that transcriptional control of Ig promoter elements can readily be observed in non-transformed B lymphocytes.