Regulation of the Na+-dependent high affinity glutamate/aspartate transporter in cultured Bergmann glia by phorbol esters
✍ Scribed by M.I. González; A. Ortega
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 96 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
The effects of phorbol 12-tetradecanoyl-13-acetate (TPA) and dibutyryl cAMP on the glutamate transport present in chick Bergmann glial cell (BGC) cultures were examined. TPA produced a significant decrease in [ 3 H]-D-aspartate uptake, while dibutyryl cAMP treatment elicited a slight reduction in the transport. This effect was dose and time dependent and sensitive to staurosporine, a Ca 2؉ /diacylglyceroldependent protein kinase C (PKC) inhibitor. Longterm exposure of the culture to TPA results in a dramatic fall of the transporter activity and a decrease in the amount of the transporter protein. These findings suggest that PKC is involved in transport modulation and possibly in the regulation of the transporter gene expression.