𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Regulation of human cripto-1 gene expression by TGF-β1 and BMP-4 in embryonal and colon cancer cells

✍ Scribed by Mario Mancino; Luigi Strizzi; Christian Wechselberger; Kazuhide Watanabe; Monica Gonzales; Shin Hamada; Nicola Normanno; David S. Salomon; Caterina Bianco

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
483 KB
Volume
215
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Human Cripto‐1 (CR‐1) is a cell membrane protein that is overexpressed in several different types of human carcinomas. In the present study we investigated the mechanisms that regulate the expression of CR‐1 gene in cancer cells. We cloned a 2,481 bp 5′‐flanking region of the human CR‐1 gene into a luciferase reporter vector and transfected NTERA‐2 human embryonal carcinoma cells and LS174‐T colon cancer cells to test for promoter activity. Activity of CR‐1 promoter in both cell lines was modulated by two TGF‐β family members, TGF‐β1 and BMP‐4. In particular, TGF‐β1 significantly up‐regulated CR‐1 promoter activity, whereas a dramatic reduction in CR‐1 promoter activity was observed with BMP‐4 in NTERA‐2 and LS174‐T cells. Changes in the CR‐1 promoter activity following TGF‐β1 and BMP‐4 treatments correlated with changes in CR‐1 mRNA and protein expression in NTERA‐2 and LS174‐T cells. We also identified three Smad binding elements (SBEs) within the CR‐1 promoter and point mutation of SBE1 (−2,197/−2,189) significantly reduced response of the CR‐1 promoter to both TGF‐β1 and BMP‐4 in NTERA‐2 and LS174‐T cells. Chromatin immunoprecipitation assay also demonstrated binding of Smad‐4 to a CR‐1 promoter DNA sequence containing SBE1 in LS174‐T cells. Finally, BMP‐4 inhibited migration of LS174‐T cells and F9 mouse embryonal carcinoma cells by downregulation of CR‐1 protein. In conclusion, these results suggest a differential modulation of CR‐1 gene expression in embryonal and colon cancer cells by two different members of the TGF‐β family. J. Cell. Physiol. 215: 192–203, 2008. © 2007 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Cell-type specific regulation of human i
Cell-type specific regulation of human interstitial collagenase-1 gene expression by interleukin-1β (IL-1β) in human fibroblasts and BC-8701 breast cancer cells
✍ Joni L. Rutter; Ulrike Benbow; Charles I. Coon; Constance E. Brinckerhoff 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 250 KB 👁 1 views

## ) is a potent cytokine that stimulates interstitial collagenase-1 (matrix metalloproteinase-1; MMP-1). In this study, we compared the mechanism(s) by which IL-1b induces collagenase gene expression in two very different cells, normal human foreskin fibroblasts (HFFs) and an aggressive breast ca

Bone marrow stroma cells regulate TIEG1
Bone marrow stroma cells regulate TIEG1 expression in acute lymphoblastic leukemia cells: Role of TGFβ/BMP-6 and TIEG1 in chemotherapy escape
✍ Guri Døsen-Dahl; Else Munthe; Marit Kveine Nygren; Heidi Stubberud; Marit E. Hys 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 French ⚖ 367 KB

## Abstract The bone marrow microenvironment regulates early B lymphopoiesis and protects leukemia cells against chemotherapy treatment, thus the microenvironment may serve as a sanctuary site for these cells. Yet, few factors that contribute to this process are known. We have explored the role of

Down-regulation of protein and mRNA expr
Down-regulation of protein and mRNA expression for transforming growth factor-β (TGF-β1) type I and type II receptors in human prostate cancer
✍ Yanping Guo; Stephen C. Jacobs; Natasha Kyprianou 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 298 KB 👁 1 views

Transforming growth factor-b (TGF-b1) is a potent negative regulator of cell growth that transduces signals through interactions with type I and II receptors. Abnormal expression and mutational alterations of these receptors have been observed in several human malignancies. In this study, we investi

Expression of the α9β1 integrin in human
Expression of the α9β1 integrin in human colonic epithelial cells: Resurgence of the fetal phenotype in a subset of colon cancers and adenocarcinoma cell lines
✍ Nuria Basora; Nathalie Desloges; Qing Chang; Yamina Bouatrouss; Jean Gosselin; J 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 French ⚖ 201 KB 👁 1 views

Cell-matrix interactions are thought to be of critical importance in the regulation of various cell functions, including proliferation, migration and control of gene expression. The integrins, a large family of specific receptors for the macromolecules of the extracellular matrix, are important medi

Expression of insulin-like growth factor
Expression of insulin-like growth factor binding protein-3 (IGFBP-3) in human keratinocytes is regulated by EGF and TGFβ1
✍ Stephanie R. Edmondson; Mari M. Murashita; Vincenzo C. Russo; Christopher J. Wra 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 195 KB 👁 2 views

Insulin-like growth factor-I (IGF-I) is essential for normal epidermal homeostasis; however, the role of IGF binding proteins (IGFBPs), regulators of IGF action, remains unclear. Here we examine the regulation of human keratinocyte-produced IGFBPs by epidermal growth factor (EGF), transforming growt