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Expression of the α9β1 integrin in human colonic epithelial cells: Resurgence of the fetal phenotype in a subset of colon cancers and adenocarcinoma cell lines

✍ Scribed by Nuria Basora; Nathalie Desloges; Qing Chang; Yamina Bouatrouss; Jean Gosselin; Jacques Poisson; Dean Sheppard; Jean-Francçois Beaulieu

Publisher
John Wiley and Sons
Year
1998
Tongue
French
Weight
201 KB
Volume
75
Category
Article
ISSN
0020-7136

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✦ Synopsis

Cell-matrix interactions are thought to be of critical importance in the regulation of various cell functions, including proliferation, migration and control of gene expression. The integrins, a large family of specific receptors for the macromolecules of the extracellular matrix, are important mediators of these interactions. The integrin ␣9␤1 is one of the integrins whose expression is restricted to specialized tissues. Its exact function is unknown. In the present study, we have analyzed expression of the ␣9 subunit in human colonic epithelial cells by indirect immuno-fluorescence and Western and Northern blots. In normal intact tissues, the antigen was detected at the basolateral domain of epithelial cells in colonic glands at the fetal stage but was absent in adults. Strong staining was detected constitutively in contractile cells at both stages. In adenocarcinomas, the ␣9 subunit was detected at the basolateral domain of epithelial cells in 6 of the 10 tumors tested, while a reduction of the staining was observed in the subepithelial myofibroblasts in parallel with peri-glandular stroma disorganization. The potential for colon adenocarcinoma cells to express the integrin ␣9 subunit was confirmed at both the protein and transcript levels in Caco-2 and T84 cell lines, 2 well-characterized cell lines known to exhibit polarization features. The 5 other cell lines tested were negative for expression of the ␣9 subunit. Taken together, our observations suggest that the ␣9 integrin subunit is subject to an onco-fetal pattern of expression in human colonic epithelium.

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