Regulation of glutamine synthetase in L cells by cortisol andl-glutamine
β Scribed by Paul R. Barnes; Robert T. Hersh; Paul A. Kitos
- Book ID
- 112796596
- Publisher
- Society for In Vitro Biology
- Year
- 1974
- Tongue
- English
- Weight
- 780 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1475-2689
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π SIMILAR VOLUMES
Metabolite control of glutamine synthetase expression (by glutamine) was studied in L6 skeletal muscle cells. Depletion of glutamine from the culture medium for 24 hours resulted in a 3-4-fold increase in glutamine synthetase activity. This effect was blocked by cycloheximide but not by actinomycin
Glutamine-synthetase (GS; EC 6.3.1.2) activity and protein levels were measured in crude extracts from Monoraphidium braunii N~iegeli, strain 202-7d, cultures grown under different nitrogen sources. Only ammonium and L-glutamine promoted a partial enzyme inactivation, which, in the case of L-glutami