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Regulation and Modulation of pH in the Brain

✍ Scribed by CHESLER, M.


Book ID
125448086
Publisher
The American Physiological Society
Year
2003
Tongue
English
Weight
413 KB
Volume
83
Category
Article
ISSN
0031-9333

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Intracellular pH (pH,) and the mechanisms of pH, regulation have been investigated in cultured microglial cells from mouse brain using the pH-sensitive fluorescent dye 2',7'-bis-(2-carboxyethyl)-5-(6)-carboxyfluorescein (BCECF). Cells were acidified by a pulse of NH,+ (4-5 min; 20 mM) and the subseq