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Regulation of Extra- and Intracellular pH in the Brain in Severe Hypoglycemia

✍ Scribed by Pelligrino, Dale; Siesjö, Bo K.

Book ID
109869362
Publisher
Nature Publishing Group
Year
1981
Tongue
English
Weight
443 KB
Volume
1
Category
Article
ISSN
0271-678X

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Intracellular pH (pH,) and the mechanisms of pH, regulation have been investigated in cultured microglial cells from mouse brain using the pH-sensitive fluorescent dye 2',7'-bis-(2-carboxyethyl)-5-(6)-carboxyfluorescein (BCECF). Cells were acidified by a pulse of NH,+ (4-5 min; 20 mM) and the subseq