Regioselective synthesis of trisaccharides by use of a reversed hydrolysis activity of α- and β-d-galactosidase

✍ Scribed by Katsumi Ajisaka; Hiroshi Fujimoto

• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 504 KB
• Volume: 185
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(89)84029-7

✦ Synopsis

Incubation of a solution containing u-galactose and a high concentration of sucrose in the presence of a-D-galactosidase from M. vinacea afforded raffinose and planteose in a ratio of -3:2. On the other hand, circulation of a solution of D-g&iCtOSe and sucrose through a reaction system consisting of columns, in series? of immobilized cu-D-galactosidase and activated carbon gave only raffinose, eluted from the activated carbon column in a 17.6% yield. Similarly, only isoraffinnse was obtained in 10.6% yield by the continuous method using an immobilized column of /3-D-galactosidase from E. coli. INTRODlJCTION