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Regions of the cloned Vibrio cholerae rfb genes needed to determine the Ogawa form of the O-antigen

โœ Scribed by Morona, Renato ;Matthews, MaryS. ;Morona, JudyK. ;BrownBrownBrown, Melissa H.

Book ID
104689803
Publisher
Springer
Year
1990
Tongue
English
Weight
988 KB
Volume
224
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

The O-antigen of the lipopolysaccharides of Vibrio cholerae 01 can exist in two forms termed Inaba and Ogawa. We used a complementation system to demonstrate that the Ogawa phenotype is dominant over the Inaba phenotype. By using a set of deletions affecting the Ogawa rfb genes, we identified two regions which are needed to confer the Ogawa phenotype. In vitro mutagenesis of the cloned Ogawa rfb genes resulted in the isolation of variants with the Inaba phenotype. The results are interpreted with respect to previous studies demonstrating interconversion between the two forms of the V. cholerae O-antigen.

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Studies on the partial structure of the
Studies on the partial structure of the O-antigen of Vibrio cholera ogawa G-2102
โœ Mita Majumder; Amal K. Mukherjee; Bhakti Guha Thakurta; Archana Dutta; Dipti Sas ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 536 KB

Detailed information was obtained regarding the partial structure of the lipopolysaccharide (LPS), containing glucose, glucuronic acid, 2-amino-2-deoxy-glucose, L-glycero-D-gluco-heptose, and small proportions of L-glycero-D-manno-heptose, mannose, and galactose, isolated from Vibrio cholera Ogawa G