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Regenerable affinity chromatography support

✍ Scribed by V.K. Joshi; K.M. Shahani; A. Kilara; Fred W. Wagner

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
563 KB
Volume
176
Category
Article
ISSN
1873-3778

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✦ Synopsis

A derivative of Sepharose 4B,p-(N-acetyl-r_-tyrosineazo)benzamidoethyl Sepharose 4B, was synthesized and used for the selective immobilization of proteins. The azo bond of this derivative can be reduced to liberate p-aminobenzamidoethyl Sepharose 4B, which can be diazotized and recoupled to N-acetyl-r_-tyrosine and subsequently to a fresh preparation of protein. This regenerable affinity chromatography support was regenerated successively through five cycles without detectable loss of functional groups.

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