Diazotizable supports of potential interest as affinity chromatography matrices

✍ Scribed by M. J. Beneš; J. Lenfeld; O. Přádová

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 437 KB
• Volume: 9
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/(sici)1099-1352(199634/12)9:5/6<701::aid-jmr325>3.0.co;2-d

✦ Synopsis

Immobilization of affinity ligands, proteins, enzymes and other functional groups by azo coupling is based on the high reactivity of the support-carrying dimnium groups towards both low-and high-molecular weight compounds containing certain groupings such as phenols, imidazole and some other heterocycles, thiols and amines.

The precursor of diazonium group is a diazotizable amine on the matrix. Remarkable progress in its preparation was achieved by application of (4-amino-phenyl)-(2-sulphatonryethyl) sulphone-type reagents for functionalization of the matrix. A similar type of amine precursor is prepared by the reaction of monosubstituted sulphanilamide with epoxide-containing matrix. The presence of a SO2 group in the para position to diazonium group of these supports (after diazotization) enhances reactivity in azo coupling.

'Reversed' azo coupling is the reaction of a matrix containing functional groups capable of reacting with diazonium groups of a ligand. Preparation of a suitable matrix and examples of diazothble ligands are given.