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Refractometric detector for a microcolumn high-performance size-exclusion and micro gel chromatograph

✍ Scribed by Maxim L. Alexandrov; Boris G. Belenkii; Vladimir A. Gotlib; Jeno E. Kever

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
500 KB
Volume
4
Category
Article
ISSN
1040-7685

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✦ Synopsis

Abstract

It is shown that an interference‐polarizing refractometric detector makes it possible to use microcolumn size‐exclusion chromatography (MSEC) columns with inner diameters of 0.2 to 0.5 mm and ensures a detection limit (at a signal to noise ratio of 3) of 8 × 10^−8^ refractive index units.

Taking into account the above requirements, a micro‐gel chromatograph with a refractometric (interference‐polarizing) detector was developed. Teflon columns gave reproducibilities (the mean square deviation at the 95% confidence level) of retention times and signal amplitudes of 0.2% and 0.5%, respectively, which ensures reproducibilities of molecular weight determinations within about 2 to 4%. Examples of polymer and protein analyses carried out on this chromatograph are given. It is suggested that a micro‐gel chromatograph can be developed for fused‐silica capillary columns with inner diameters of 0.2 mm. This would permit analysis of approximately 10 pg of polymer.

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