✦ LIBER ✦

A miniaturized evaporative light scattering detector for application with packed microcolumn high-performance liquid chromatography

✍ Scribed by Magnus B. O. Andersson; Lars G. Blomberg

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 86 KB
Volume: 10
Category: Article
ISSN: 1040-7685
DOI: 10.1002/(sici)1520-667x(1998)10:3<249::aid-mcs2>3.0.co;2-g

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✦ Synopsis

A miniaturized evaporative light scattering detector ELSD compatible Ž . with packed microcolumn high-performance liquid chromatography HPLC has been developed. In such a detector, it is particularly important that an efficient nebulization of the column effluent is achieved. Therefore, the geometry of the nebulizer has been optimized. Detector performance was demonstrated for ace-tonitrile᎐acetone at 5᎐100 L min y1 as mobile phase, a packed microcolumn, 0.7 mm i.d. was used. Isocratic and gradient elutions were performed. Detection limit was lower than 8 ng. Linear range was 30᎐300 ng. Quantitative analysis of cohune oil components separated on a C-18 column using gradient elution was examined.

Ž . The relative standard deviation RSD in peak areas was 1.9᎐3.7% for large peaks. Minor peaks gave higher RSD.

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