✦ LIBER ✦
Separation and Quantitation of Bile Acids Using an Isocratic Solvent System for High Performance Liquid Chromatography Coupled to an Evaporative Light Scattering Detector
✍ Scribed by Enrique C. Torchia; Eric D. Labonté; Luis B. Agellon
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 80 KB
- Volume
- 298
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids.