By adding salts (sodium chloride, ammonium sulfate), it is possible to induce phase separation in membraneprotein solubilisates containing Triton \(\mathrm{X}-100\) or Nonidet \(\mathrm{P}-40\) at temperatures between 0 and \(20^{\circ} \mathrm{C}\). Other nonionic detergents of the Brij, Lubrol, an
Refractive index-based determination of detergent concentration and its application to the study of membrane proteins
β Scribed by Pavel Strop; Axel T. Brunger
- Book ID
- 111752530
- Publisher
- Cold Spring Harbor Laboratory Press
- Year
- 2005
- Tongue
- English
- Weight
- 194 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0961-8368
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π SIMILAR VOLUMES
The conditions for obtaining highly concentrated salt-free protein solutions have been examined. It was found that a convenient and rapid method consists of combining ion-exchange and gel-filtration chromatography in one column. We observed that a volume ratio of Sephadex G-25 to ion exchanger of 2.
## Synopsis llilferences in refractive index between deuterated and normal solvents have been determined using a conventional gel permeation chromatograph equipped with a differential refractometer detector. Three molecular systems have been studied, namely, walerdeuterium oxide, acetone-acetone (
The Critically Acclaimed Laboratory Standard, Methods In Enzymology, Is One Of The Most Highly Respected Publications In The Field Of Biochemistry. Since 1955, Each Volume Has Been Eagerly Awaited, Frequently Consulted, And Praised By Researchers And Reviewers Alike. The Series Contains Much Materia