Deuterium isotope effect on the refractive index of liquids and its application to permeation chromatography

✍ Scribed by Yvon P. Carignan; Edward V. Turngren

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 253 KB
• Volume: 15
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.1971.070150619

✦ Synopsis

Synopsis

llilferences in refractive index between deuterated and normal solvents have been determined using a conventional gel permeation chromatograph equipped with a differential refractometer detector. Three molecular systems have been studied, namely, walerdeuterium oxide, acetone-acetone (dc), and tetrahydrofuran-tetrahydrofuran (ds). A plot of the signal intensity versus the concentration of the deuterated solvent in the normal solvent gave a linear relationship for all three systems studied. The effect of deuteration is found to result in a lowering of the refractive index which is a t least three orders of magnitude larger than the sensitivity of the differential refractometer. Consequently, for permeation chromatographic studies, the addition of 1% or less of the deuterated solvent in the injection sample would be more than sufficient to produce a peak characteristic of the mobile phase.