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Refolding of Thermally and Urea-Denatured Ribonuclease A Monitored by Time-Resolved FTIR Spectroscopy †

✍ Scribed by Reinstädler, Diane; Fabian, Heinz; Backmann, Jan; Naumann, Dieter


Book ID
127057489
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
386 KB
Volume
35
Category
Article
ISSN
0006-2960

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New structural insights into the refoldi
✍ Diane Reinstädler; Heinz Fabian; Dieter Naumann 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 305 KB 👁 2 views

To get new structural insights into different phases of the renaturation of ribonuclease T1 (RNase T1), the refolding of the thermally unfolded protein was initiated by rapid temperature jumps and detected by time-resolved Fourier-transform infrared spectroscopy. The characteristic spectral changes