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Reductive Heme-Dependent Activation of the N -Oxide Prodrug AQ4N by Nitric Oxide Synthase

✍ Scribed by Nishida, Clinton R.; Ortiz de Montellano, Paul R.

Book ID
126254220
Publisher
American Chemical Society
Year
2008
Tongue
English
Weight
144 KB
Volume
51
Category
Article
ISSN
0022-2623

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1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med