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Reduction of nuclear DNA contamination in the analysis of chloroplast DNA with restriction endonucleases

✍ Scribed by Jian-Wei Liu; Ray J. Rose


Book ID
112822073
Publisher
Springer
Year
1993
Tongue
English
Weight
541 KB
Volume
11
Category
Article
ISSN
0735-9640

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Yeast ribosomal DNA (rDNA) was digested with the restriction enzymes Hind III, Hind II and a mixture of Hind II and Hind III. The cleavage products were analyzed by electrophoresis on 1.5% agarose gels. Several distinct bands could be observed, which are derived from the redundant ribosomal transcri