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Reduction of escapee formation in flow cytometric analysis of lymphocyte subsets

✍ Scribed by Harry E. Prince; Joanne York; D.Kip Kuttner

Book ID
118903025
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
762 KB
Volume
177
Category
Article
ISSN
0022-1759

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During immunostaining, human lymphocytes may form aggregates with activated platelets and monocytes, resulting in increased forward (FSC) and sideward (SSC) light scatter signals. Consequently, aggregated cells "escape" from the standard FSC-SSC analysis gate, thereby producing erroneous results. We