Redox modifications of spinach chloroplast fructose-1,6-bisphosphatase
β Scribed by Ye Chen; Gen-jun Xu
- Book ID
- 111741986
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 388 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1521-6543
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Using partially purified sedoheptulose-l,7bisphosphatase from spinach (Spinacia oleracea L.) chloroplasts the effects of metabolites on the dithiothreitoland Mg 2 +-activated enzyme were investigated. A screening of most of the intermediates of the Calvin cycle and the photorespiratory pathway showe
A rapid procedure for the purification of fructose-l,6-bisphosphate aldolase from spinach chloroplasts is presented which involves two steps; precipitation of bulk protein with polyethylene glycol and partitioning of remaining soluble protein in aqueous two-phase systems. A 94% pure preparation is o