𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Rapid purification of fructose-1,6-bisphosphate aldolase from spinach chloroplasts

✍ Scribed by Lars-Olof Persson

Publisher
Springer
Year
1988
Tongue
English
Weight
421 KB
Volume
15
Category
Article
ISSN
0166-8595

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid procedure for the purification of fructose-l,6-bisphosphate aldolase from spinach chloroplasts is presented which involves two steps; precipitation of bulk protein with polyethylene glycol and partitioning of remaining soluble protein in aqueous two-phase systems. A 94% pure preparation is obtained within 6h with a yield of 19%. A marked difference in the partition behaviour of the aldolase activity from whole leaf tissue suggested that the procedure is less efficient when leaf extract is used as starting material.

πŸ“œ SIMILAR VOLUMES

Fructose-1,6-bisphosphate aldolase from
Fructose-1,6-bisphosphate aldolase from Vibrio marinus, a psychrophilic marine bacterium
✍ L. P. Jones; Dr. R. Y. Morita; R. R. Becker πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 607 KB

Fructosr-1,6-bispliosphate aldolase (Pru-P,A) from a psychrophilic marine bacterium n a s found to be Class 11 aldolase based on activation by I<+, activation by divaleiit cations, iiiartivatioii by EDTA, low molecular weight. and similar values for K , , I'II~~x, and ARRIIENIUS activation energy. T

A one-step procedure for the isolation o
A one-step procedure for the isolation of fructose 1,6-bisphosphatase and fructose 1,6-bisphosphate aldolase from rabbit liver
✍ Hiroshi Kido; Alberto Vita; B.L. Horecker πŸ“‚ Article πŸ“… 1980 πŸ› Elsevier Science 🌐 English βš– 604 KB

Adsorption to blue dextran-Sepharose and successive elution with AMP and fructose 1,6-bisphosphate provides a one-step procedure for the simultaneous purification of fructose 1,6-bisphosphatase and fructose 1,Cbisphosphate aldolase from rabbit liver homogenates. The procedure can be completed in a s