Recovery of poly-3-hydroxyalkanoic acid granules by a surfactant-hypochlorite treatment
β Scribed by J. A. Ramsay; E. Berger; B. A. Ramsay; C. Chavarie
- Book ID
- 104648387
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 406 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0951-208X
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β¦ Synopsis
When Alcaliaenes eutrophus biomass was treated with a surfactant and then washed with hypochlorite, the recovered poly-3-hydroxyalkanoic acid (PHA) granules were 97 to 98% pure with a molecular weight (4) between 730,000 and 790,000, depending on the surfactant used.
When treated with only surfactant, the q was slightly higher than that obtained with the surfactant-hypochlorite treatment but the purity was 10% lower. PHA of higher purity but lower M,, was obtained with just a hypochlorite treatment.
π SIMILAR VOLUMES
Recombinant strains of Pseudomonas oleovorans, which harbour the poly(3-hydroxybutyrate)-bio- synthetic genes of Alcaligenes eutrophus, accumulated poly(hydroxyalkanoates), composed of 3-hydroxybutyrate(3HB), 3-hydroxyhexanoate (3HHx) and 3-hydroxyoctanoate (3HO), up to 70% of the cell dry weight if