Reconstitution of the Cpx signaling system from cell-free synthesized proteins
β Scribed by Marika Miot; Jean-Michel Betton
- Publisher
- Elsevier
- Year
- 2011
- Tongue
- English
- Weight
- 281 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1871-6784
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Two modifications are introduced to convert the Escherichia coli cell-free extract ("S30") into a high efficiency system for coupled transcription/translation of exogenously added genes. (a) The ribosome fraction collected from the S30 by ultracentrifugation is used. It contains all the proteins nec
## Abstract Cellβfree systems from __Catharanthus roseus__ plants are utilized for various studies relating to the biosynthesis of indole alkaloids. Tryptamine (**5**) and secologanin (**6**), two fundamental building units, are shown to be incorporated into the alkaloid vindoline (**7**). In anoth