Recombinational analysis of OXI1 mutants and preliminary analysis of their translation products inS. cerevisiae

✍ Scribed by Anna Kruszewska; Barbara Szcześniak; Maurice Claisse

• Book ID: 104777254
• Publisher: Springer-Verlag
• Year: 1980
• Tongue: English
• Weight: 647 KB
• Volume: 2
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00445693

✦ Synopsis

Recombinational analysis of oxil mutants was performed using a and a mutant strains with the same mitochondrial and nuclear backgrounds, derived from strain 777-3A.

In spite of minor inconsistencies the overall map of oxil mutations can be constructed on the basis of wildtype recombinant frequencies in the two-point oxil-x oxil -crosses. The frequencies of wild-type recombinants varied in a wide range from 0.003% to 16%, reaching the maximal values expected for unlinked mitochondrial markers. No distinct clusters of mutants were observed.

The analysis of translation products of oxil mutants showed that all but one of the oxil mutants studied are connected with the conspicuous changes of the polypeptide band corresponding to subunit II ofcytochrome c oxidase in electrophoresis on polyacrylamide gels. The exceptional G565 mutant showed no conspicuous change in subunit II, but lacked subunit I of cytochrome c oxidase.

Various oxil mutants seemed to carry premature chain termination mutations. Most of them show a correlation between the length of the putative fragment of subunit II synthesized and the position on the genetic map. The direction of translation is from the V2 to the V60 mutation. The V2 mutation is proximal to cap and V60 proximal to the par locus.