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Genetic mapping and biochemical analysis of mutants in the maltose regulatory gene of theMAL1locus ofSaccharomyces cerevisiae

โœ Scribed by Michael J. Goldenthal; Marco Vanoni

Publisher
Springer
Year
1990
Tongue
English
Weight
687 KB
Volume
154
Category
Article
ISSN
0302-8933

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โœฆ Synopsis

The MAL1 locus of Saccharomyces cerevisiae comprises three genes necessary for maltose utilization: a regulatory (MALR), a maltose transport (MALT) and a maltase gene (MALS). A fine structure genetic map of the MAL1R gene was constructed and the order of mutations was confirmed by plasmid-mediated chromosomal recombination. The mutations cluster non-randomly within the 5' half of the gene, where the putative DNA binding domain of the encoded protein is located. Only mutations mal1R-22 and MAL1R-72 map in the 3' terminal half of the gene; these mutations cause a different pattern of transcriptional regulation of plasmid-borne MAL6T genes. Experiments supporting a direct involvement of the MALR-encoded protein in carbon catabolite repression of MAL gene expression are reported.

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