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Recombination following transformation of Escherichia coli by heteroduplex plasmid DNA molecules

โœ Scribed by Shing, Chang; Diana, Ho; McLaughlin, Jane R.; Sheng-Yung, Chang


Book ID
123489633
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
642 KB
Volume
29
Category
Article
ISSN
0378-1119

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Monomeric pBR322 DNA that had been linearized at its unique SalI site transformed wild-type Escherichia coli with 102 to 103 times less efficiency than CCC plasmid DNA. Dose-response experiments indicated that a single linear plasmid 'molecule' was sufficient to produce a transformant. Transformatio