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Transformation of Escherichia coli C600 by plasmid DNA at different phases of growth

โœ Scribed by Moira G.M. Brown; A. Weston; J.R. Saunders; G.O. Humphreys


Book ID
109310260
Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
220 KB
Volume
5
Category
Article
ISSN
0378-1097

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Monomeric pBR322 DNA that had been linearized at its unique SalI site transformed wild-type Escherichia coli with 102 to 103 times less efficiency than CCC plasmid DNA. Dose-response experiments indicated that a single linear plasmid 'molecule' was sufficient to produce a transformant. Transformatio