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Recombinase-mediated mouse transgenesis by intracytoplasmic sperm injection

✍ Scribed by Takehito Kaneko; Stefan Moisyadi; Ryota Suganuma; Barbara Hohn; Ryuzo Yanagimachi; Pawel Pelczar

Book ID
116910538
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
217 KB
Volume
64
Category
Article
ISSN
0093-691X

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## Abstract We demonstrate enhanced transgenesis in mice by intracytoplasmic injection of envelope‐free lentivirus. Envelope‐free lentivirus carrying the green fluorescent protein (GFP) gene under the control of the ubiquitin promoter (LVU‐GFP) was microinjected into the cytoplasm of mouse zygotes