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Mammalian Transgenesis by Intracytoplasmic Sperm Injection

โœ Scribed by Perry, A. C.


Book ID
121161035
Publisher
American Association for the Advancement of Science
Year
1999
Tongue
English
Weight
659 KB
Volume
284
Category
Article
ISSN
0036-8075

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## Abstract We demonstrate enhanced transgenesis in mice by intracytoplasmic injection of envelopeโ€free lentivirus. Envelopeโ€free lentivirus carrying the green fluorescent protein (GFP) gene under the control of the ubiquitin promoter (LVUโ€GFP) was microinjected into the cytoplasm of mouse zygotes