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Recombinant Strategies for Rapid Purification of Catalytic Subunits of cAMP-Dependent Protein Kinase

✍ Scribed by Wolfram Hemmer; Maria McGlone; Susan S. Taylor


Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
286 KB
Volume
245
Category
Article
ISSN
0003-2697

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A peptide affinity inactivator, Ac-Leu-Arg-Arg-Ala-(BrAc)Om-Leu-Gly, was used as a tool t o probe for active site residues in the catalytic subunit of bovine CAMP-dependent protein kinase. The peptide inactivated the catalytic subunit in an active site-directed and monophasic manner with a first-ord