Recombinant protein purification by self-cleaving aggregation tag
โ Scribed by Wu, Wan-Yi ;Mee, Courtney ;Califano, Filomena ;Banki, Reza ;Wood, David W
- Book ID
- 115502755
- Publisher
- Nature Publishing Group
- Year
- 2006
- Tongue
- English
- Weight
- 218 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1750-2799
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## Abstract The biological revolution, spurred on by the completion of the human genome project, will increase the demand for inexpensive and reliable methods for protein expression and purification. Over the past 20 years, several technologies have been developed where target proteins or expressio
## Abstract We have combined Invitrogen's Gatewayยฎ cloning technology with selfโcleaving purification tags to generate a new system for rapid production of recombinant protein products. To accomplish this, we engineered our previously reported ฮIโCM cleaving intein to include a Gateway cloning reco