Rapid cloning and purification of protei
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Alison R. Gillies; Judy F. Hsii; Seachol Oak; David W. Wood
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Article
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2008
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John Wiley and Sons
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English
⚖ 506 KB
## Abstract We have combined Invitrogen's Gateway® cloning technology with self‐cleaving purification tags to generate a new system for rapid production of recombinant protein products. To accomplish this, we engineered our previously reported ΔI‐CM cleaving intein to include a Gateway cloning reco