A transducing phage carrying some of the genes (men) defining the early stages of menaquinone biosynthesis was isolated from a pool of recombinant lambda phages that had been constructed from R.HindIII digests of E. coli DNA and the corresponding insertion vector. The lesions of menB and menC mutant
Recombinant plasmids containing menaquinone biosynthetic genes of Escherichia coli
β Scribed by Duncan J. Shaw; E.Clare Robinson; R. Meganathan; Ronald Bentley; John R. Guest
- Book ID
- 109312209
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 325 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0378-1097
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Plasmid pserB59-1 carries the E. coli serB gene on a 5.2 kb BamHI fragment cloned into the BamHI site of plasmid pBR322. The results of genetic and biochemical experiments established that a functional serB gene is contained in the fragment. The location of the serB gene within the insert was determ