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Recombinant human cytochrome P450 monooxygenases for drug metabolite synthesis

✍ Scribed by Kirsten Schroer; Matthias Kittelmann; Stephan Lütz

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
636 KB
Volume
106
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

Cytochrome P450 monooxygenases (CYPs) are important enzymes in the metabolism of xenobiotics. Therefore, several approaches to clone and overexpress the human isoforms have been made. In addition to microsomes or S9 preparations, these recombinant human isoforms have found diverse application in drug development. We discuss and give examples of the use of bacterial whole cell systems with rec. human CYPs for the preparative scale synthesis of drug metabolites. Biotechnol. Bioeng. 2010;106: 699–706. © 2010 Wiley Periodicals, Inc.

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