A Diversified Library of Bacterial and Fungal Bifunctional Cytochrome P450 Enzymes for Drug Metabolite Synthesis

A Panel of Cytochrome P450 BM3 Variants

A Panel of Cytochrome P450 BM3 Variants to Produce Drug Metabolites and Diversify Lead Compounds

✍ Andrew M. Sawayama; Michael M. Y. Chen; Palaniappan Kulanthaivel; Ming-Shang Kuo 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 251 KB

## Abstract Herein we demonstrate that a small panel of variants of cytochrome P450 BM3 from __Bacillus megaterium__ covers the breadth of reactivity of human P450s by producing 12 of 13 mammalian metabolites for two marketed drugs, verapamil and astemizole, and one research compound. The most acti