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Recombinant full-length murine prion protein, mPrP(23–231): purification and spectroscopic characterization

✍ Scribed by Simone Hornemann; Carsten Korth; Bruno Oesch; Roland Riek; Gerhard Wider; Kurt Wüthrich; Rudi Glockshuber


Book ID
117108022
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
530 KB
Volume
413
Category
Article
ISSN
0014-5793

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Purification and spectroscopic character
✍ Vladimir V. Bamm; Mumdooh A.M. Ahmed; Vladimir Ladizhansky; George Harauz 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 691 KB

## Abstract A recombinant form of the murine Golli‐myelin basic protein (MBP) isoform BG21 (rmBG21) has been expressed in __E. coli__, and isolated to 96% purity via metal chelation chromatography. Characteristic yields were 6–8 mg protein per liter of culture in either minimal M9 or standard Luria