This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications.
Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!
Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as
plasmid biology
nucleic acid biochemistry
restriction enzymes
cloning strategies
gel electrophoresis
southern and northern blotting
preparation of probes
phage lambda biology
cosmids and genome analysis
cloned gene expression
polymerase chain reaction
conventional and automated DNA sequencing
site-directed mutagenesis
and more!
Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.