Recombinant DNA methodology

✍ Scribed by Ray Wu; Lawrence Grossman; Kivie Moldave

• Publisher: Academic Press, , Elsevier Inc
• Year: 1989
• Tongue: English
• Leaves: 751
• Series: Selected methods in enzymology
• Edition: 1
• Category: Library

✦ Synopsis

Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry.
Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher.

Key Features
Enzymes in DNA research
DNA isolation, hybridization, and cloning
DNA sequence analysis
cDNA cloning
Gene products
Identification of cloned genes and mapping of genes
Monitoring cloned gene expression
Cloning and transferring of genes into yeast cells
Cloning and transferring of genes into plant cells
Cloning and transferring of genes into animal cells
Site-directed mutagenesis
Protein engineering
* Expression vectors

✦ Table of Contents

Content:
SELECTED METHODS IN ENZYMOLOGY, Page ii
Front Matter, Page iii
Copyright, Page iv
List of Contributors, Pages ix-xiii
Preface, Page xiii, RAY WU, LAWRENCE GROSSMAN, KIVIE MOLDAVE
Contents of Methods in Enzymology Volumes 68, 100, 101, 153, 154, and 155, Pages xv-xxxi
1 - DNA-Joining Enzymes: A Review, Pages 3-24, N. PATRICK HIGGINS, NICHOLAS R. COZZARELLI
2 - Guide to the Use of Type II Restriction Endonucleases, Pages 25-60, ROY FUCHS, ROBERT BLAKESLEY
3 - Site-Specific Cleavage of DNA at 8-, 9-, and 10-bp Sequences, Pages 61-71, MICHAEL MCCLELLAND
4 - Exonuclease III: Use for DNA Sequence Analysis and in Specific Deletions of Nucleotides, Pages 73-109, LI-HE GUO, RAY WU
5 - Gel Electrophoresis of Restriction Fragments, Pages 113-137, EDWIN SOUTHERN
6 - Purification, Specific Fragmentation, and Separation of Large DNA Molecules, Pages 139-157, CASSANDRA L. SMITH, CHARLES R. CANTOR
7 - Orthogonal-Field-Alternation Gel Electrophoresis, Pages 159-173, GEORGES F. CARLE, MAYNARD V. OLSON
8 - A Rapid Alkaline Extraction Method for the Isolation of Plasmid DNA, Pages 175-187, H.C. BIRNBOIM
9 - Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction, Pages 189-204, KARY B. MULLIS, FRED A. FALOONA
10 - Escherichia coli Plasmids Packageable in Vitro in λ Bacteriophage Particles, Pages 207-224, JOHN COLLINS
11 - Production of Single-Stranded Plasmid DNA, Pages 225-233, JEFFREY VIEIRA, JOACHIM MESSING
12 - High-Efficiency Cloning of Full-Length cDNA; Construction and Screening of cDNA Expression Libraries for Mammalian Cells, Pages 235-260, H. OKAYAMA, M. KAWAICHI, M. BROWNSTEIN, F. LEE, T. YOKOTA, K. ARAI
13 - Transformation and Preservation of Competent Bacterial Cells by Freezing, Pages 261-265, D.A. MORRISON
14 - Plasmid Screening at High Colony Density, Pages 267-275, DOUGLAS HANAHAN, MATTHEW MESELSON
15 - New Bacteriophage Lambda Vectors with Positive Selection for Cloned Inserts, Pages 277-293, JONATHAN KARN, SYDNEY BRENNER, LESLIE BARNETT
16 - λ Phage Vectors—EMBL Series, Pages 295-307, A.M. FRISCHAUF, N. MURRAY, H. LEHRACH
17 - λgt 11: Gene Isolation with Antibody Probes and Other Applications, Pages 309-330, MICHAEL SNYDER, STEPHEN ELLEDGE, DOUGLAS SWEETSER, RICHARD A. YOUNG, RONALD W. DAVIS
18 - One-Step Gene Disruption in Yeast, Pages 331-340, RODNEY J. ROTHSTEIN
19 - Cloning Regulated Yeast Genes from a Pool of lacZ Fusions, Pages 341-357, STEPHANIE W. RUBY, JACK W. SZOSTAK, ANDREW W. MURRAY
20 - Selection Procedure for Isolation of Centromere DNAs from Saccharomyces cerevisiae, Pages 359-365, LOUISE CLARKE, CHU-LAI HSIAO, JOHN CARBON
21 - Construction of High Copy Yeast Vectors Using 2-μm Circle Sequences, Pages 367-385, JAMES R. BROACH
22 - Improved Vectors for Plant Transformation: Expression Cassette Vectors and New Selectable Markers, Pages 387-410, S.G. ROGERS, H.J. KLEE, R.B. HORSCH, R.T. FRALEY
23 - β-Galactosidase Gene Fusions for Analyzing Gene Expression in Escherichia coli and Yeast, Pages 413-428, MALCOLM J. CASADABAN, ALFONSO MARTINEZ-ARIAS, STUART K. SHAPIRA, JOANY CHOU
24 - The Use of pKC30 and Its Derivatives for Controlled Expression of Genes, Pages 429-434,436-444, MARTIN ROSENBERG, YEN-SEN HO, ALLAN SHATZMAN
25 - Expression of Heterologous Unfused Protein in Escherichia coli, Pages 445-460, ERIK REMAUT, ANNE MARMENOUT, GUUS SIMONS, WALTER FIERS
26 - Expression and Secretion of Foreign Proteins in Escherichia coli, Pages 461-476, GUY D. DUFFAUD, PAUL E. MARCH, MASAYORI INOUYE
27 - Yeast Promoters and lacZ Fusions Designed to Study Expression of Cloned Genes in Yeast, Pages 477-487, LEONARD GUARENTE
28 - The Use of Xenopus Oocytes for the Expression of Cloned Genes, Pages 489-505, J.B. GURDON, M.P. WICKENS
29 - Expression and Secretion Vectors for Yeast, Pages 507-534, GRANT A. BITTER, KEVIN M. EGAN, RAYMOND A. KOSKI, MATTHEW O. JONES, STEVEN G. ELLIOTT, JAMES C. GIFFIN
30 - Oligonucleotide-Directed Mutagenesis of DNA Fragments Cloned into M13 Vectors, Pages 537-568, MARK J. ZOLLER, MICHAEL SMITH
31 - Oligonucleotide-Directed Construction of Mutations via Gapped Duplex DNA, Pages 569-585, WILFRIED KRAMER, HANS-JOACHIM FRITZ
32 - Rapid and Efficient Site-Specific Mutagenesis without Phenotypic Selection, Pages 587-601, THOMAS A. KUNKEL, JOHN D. ROBERTS, RICHARD A. ZAKOUR
33 - An Improved Method to Obtain a Large Number of Mutants in a Defined Region of DNA, Pages 603-618, RICHARD PINE, P.C. HUANG
34 - Mutagenesis with Degenerate Oligonucleotides: An Efficient Method for Saturating a Defined DNA Region with Base Pair Substitutions, Pages 619-629, DAVID E. HILL, ARNOLD R. OLIPHANT, KEVIN STRUHL
35 - Fusogenic Reconstituted Sendai Virus Envelopes as a Vehicle for Introducing DNA into Viable Mammalian Cells, Pages 633-652, A. VAINSTEIN, A. RAZIN, A. GRAESSMANN, A. LOYTER
36 - In Vitro Transcription: Whole-Cell Extract, Pages 653-666, JAMES L. MANLEY, ANDREW FIRE, MARK SAMUELS, PHILLIP A. SHARP
37 - Translation of Exogenous mRNAs in Reticulocyte Lysates, Pages 667-676, WILLIAM C. MERRICK
38 - Preparation of a Cell-Free Protein-Synthesizing System from Wheat Germ, Pages 677-685, CARL W. ANDERSON, J. WILLIAM STRAUS, BERNARD S. DUDOCK
39 - Direct Gene Transfer to Protoplasts of Dicotyledonous and Monocotyledonous Plants by a Number of Methods, Including Electroporation, Pages 687-709, RAYMOND D. SHILLITO, INGO POTRYKUS
40 - A Rapid Single-Stranded Cloning, Sequencing, Insertion, and Deletion Strategy, Pages 711-720, RODERIC M.K. DALE, AMY ARROW
41 - Hydroxyl Radical Footprinting: A High-Resolution Method for Mapping Protein-DNA Contacts, Pages 721-741, THOMAS D. TULLIUS, BETH A. DOMBROSKI, MAIR E.A. CHURCHILL, LAURANCE KAM
Index, Pages 743-760