Recent Insights into Inhibition, Structure, and Mechanism of Configuration-Retaining Glycosidases

✍ Scribed by Tom D. Heightman; Andrea T. Vasella

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 744 KB
• Volume: 38
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/(sici)1521-3773(19990315)38:6<750::aid-anie750>3.0.co;2-6

✦ Synopsis

Not "from above", but "from the side": Configuration-retaining β-glycosidases protonate their substrate either anti or syn to the endocyclic C1-O bond as the first step in the enzymic cleavage of the glycosidic bond (see schematic drawing). Insights into the mechanism of action of glycosidases have been gained by a combination of the synthesis of inhibitors, the study of the kinetics of their inhibition, and the analysis of the crystal structures of glycosidases and glycosidase-ligand complexes.