Reannotation of protein-coding genes based on an improved graphical representation of DNA sequence

✍ Scribed by Jia-Feng Yu; Xiao Sun

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 816 KB
• Volume: 31
• Category: Article
• ISSN: 0192-8651
• DOI: 10.1002/jcc.21500

✦ Synopsis

Abstract

Over annotation of protein coding genes is common phenomenon in microbial genomes, the genome of Amsacta moorei entomopoxvirus (AmEPV) is a typical case, because more than 63% of its annotated ORFs are hypothetical. In this article, we propose an improved graphical representation titled I‐TN (improved curve based on trinucleotides) curve, which allows direct inspection of composition and distribution of codons and asymmetric gene structure. This improved graphical representation can also provide convenient tools for genome analysis. From this presentation, 18 variables are exploited as numerical descriptors to represent the specific features of protein coding genes quantitatively, with which we reannotate the protein coding genes in several viral genomes. Using the parameters trained on the experimentally validated genes, all of the 30 experimentally validated genes and 63 putative genes in AmEPV genome are recognized correctly as protein coding, the accuracies of the present method for self‐test and cross‐validation are 100%, respectively. Twenty‐eight annotated hypothetical genes are predicted as noncoding, and then the number of reannotated protein coding genes in AmEPV should be 266 instead of 294 reported in the original annotations. Extending the present method trained in AmEPV to other entomopoxvirus genomes directly, such as Melanoplus sanguinipes entomopoxvirus (MsEPV), all of the 123 annotated function‐known and putative genes are recognized correctly as protein coding, and 17 hypothetical genes are recognized as noncoding. The present method could also be extended to other genomes with or without adaptation of training sets with high accuracy. © 2010 Wiley Periodicals, Inc. J Comput Chem 2010