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Real-time quantitative RT–PCR after laser-assisted cell picking

✍ Scribed by Fink, Ludger; Seeger, Werner; Ermert, Leander; Hänze, Jörg; Stahl, Ulrich; Grimminger, Friedrich; Kummer, Wolfgang; Bohle, Rainer M.


Book ID
109753724
Publisher
Nature Publishing Group
Year
1998
Tongue
English
Weight
130 KB
Volume
4
Category
Article
ISSN
1078-8956

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## Abstract We developed and used real‐time RT‐PCR assays to investigate how the expression of typical osteoblast‐related genes by human bone marrow stromal cells (BMSC) is regulated by (i) the culture time in medium inducing osteogenic differentiation and (ii) the previous expansion in medium enha