## Abstract The detection of disseminated tumor cells in peripheral blood from colorectal cancer patients by RTβPCR could be an attractive method for selecting patients for adjuvant therapy. We here report on realβtime RTβPCR assays (LightCycler) to quantitate potential mRNA markers. We investigate
Detection of colorectal cancer cells from feces using quantitative real-time RT-PCR for colorectal cancer diagnosis
β Scribed by Yoshikatsu Koga; Masahiro Yasunaga; Yoshihiro Moriya; Takayuki Akasu; Shin Fujita; Seiichiro Yamamoto; Takahiro Kozu; Hideo Baba; Yasuhiro Matsumura
- Book ID
- 108584333
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 247 KB
- Volume
- 99
- Category
- Article
- ISSN
- 1347-9032
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## Abstract The purpose of our study was to develop specific, sensitive, objective assays for early detection of disseminated tumour cells in patients with colorectal cancer (CRC). Carcinoembryonic antigen (CEA) and cytokeratin 20 (CK20) were chosen as markers because they are selectively expressed
Real-time RT-PCR is a relatively new technology that uses an online fluorescence detection system to determine gene expression levels. It has the potential to significantly improve detection of breast cancer metastasis by virtue of its exquisite sensitivity, high throughput capacity and quantitative