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Real-Time PCR Sensing by Thermal Conductivity Monitoring

✍ Scribed by Seungbeom Noh; Jinsang Lee; Jang-Kyoo Shin; Geunbae Lim

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
139 KB
Volume
18
Category
Article
ISSN
1040-0397

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✦ Synopsis

Abstract

Traditional PCR (Polymerase Chain Reaction) technology is based on an end‐point system which confirms the existence of a target DNA sequence by electrophoresis and UV transillumination steps. This process is labor‐intensive and time consuming. A real‐time PCR is the best alternative that can improve problems of analysis time and accuracy. Therefore, we propose a smaller, non‐optical and non‐labeling system with the advantages of an established real‐time PCR. The proposed technique utilizes a variation of the thermal conductivity of the DNA sample with a PCR cycle. We confirmed that thermal conductivity depends on amounts of PCR products.

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